Abcam, ab133113, PPAR (alpha, delta, gamma) Transcription Factor Assay Kit

Size: 96Test
Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Key facts
Detection method:Colorimetric,
Sample types:Nuclear Extracts, Suspension cells, Adherent cells,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Microplate reader

Product details:
Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR alpha, delta, or gamma are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133113 comes with a single plate that measures all three isoforms of PPAR alpha, delta, and gamma. There are enough reagents for one-third of a plate for each isoform.
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands. PPARs play pivotal roles in the regulation of lipid metabolism and homeostasis and are important indirect and direct regulators of cellular insulin sensitivity.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols