Abcam, ab133292, Anti-Tropomyosin 1 (alpha) + Tropomyosin 3 antibody [EPR5159]

Size: 10µL-TRIAL / 100µL / 1mL
Rabbit Recombinant Monoclonal TPM1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5159,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tropomyosin 1 (alpha) and Tropomyosin 3 are actin-binding proteins with key roles in stabilizing actin filaments within muscle and non-muscle cells. Often referred to as TPM1 and TPM3 these proteins have molecular masses of approximately 32 kDa and 33 kDa respectively. TPM1 and TPM3 are expressed in various tissues such as heart skeletal muscle and brain. They help maintain structural integrity of the cytoskeleton by interacting with actin filaments providing essential support for cellular functions.
Biological function summary
Tropomyosin isoforms bind along the length of actin filaments regulating their interaction with myosin and other actin-binding proteins. TPM1 and TPM3 form part of a complex that includes troponin contributing to the calcium-dependent regulation of muscle contraction. In non-muscle cells they regulate cell shape cytokinesis and intracellular transport. Through their role in stabilizing actin filaments these proteins influence many cellular processes.
Pathways
TPM1 and TPM3 are essential for muscle contraction and cytoskeletal dynamics. They play significant roles in the actin cytoskeleton and cardiac muscle contraction pathways. Tropomyosins interact with proteins like myosin and troponin to modulate muscle contraction. They ensure effective actin-myosin interaction by allowing proper binding and release cycles during contraction and relaxation.
TPM1 and TPM3 mutations have associations with conditions such as hypertrophic cardiomyopathy and nemaline myopathy. These genetic mutations can alter their ability to regulate actin filament function contributing to abnormal contraction and muscle weakness. In hypertrophic cardiomyopathy TPM1 mutations often occur alongside mutations in proteins like myosin heavy chain and cardiac myosin-binding protein C compounding the disease's effects and presenting critical challenges in cardiac function.