Abcam, ab133333, Anti-Aldolase B + Aldolase C antibody [EPR3137]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3137,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aldolase B also known as fructose-bisphosphate aldolase B and Aldolase C or fructose-bisphosphate aldolase C are key enzymes in glycolysis and gluconeogenesis pathways. These enzymes are part of the aldolase family and share high sequence homology. Aldolase B has a molecular mass of approximately 36-40 kDa and is primarily expressed in the liver whereas Aldolase C is majorly found in the brain particularly within neurons. They function to catalyze the cleavage of fructose-16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate critical steps in energy metabolism.
Biological function summary
Aldolase B and C perform major roles in cellular energy production and metabolism. Aldolase B facilitates the conversion of fructose into glycolytic intermediates aiding glycogen synthesis and gluconeogenesis in the liver. Aldolase C contributes to glucose metabolism in the brain supporting neural activities and functions. While they do not form stable complexes these enzymes occasionally interact transiently with other metabolic proteins to fulfill cellular functions.
Pathways
Aldolase B and C integrate into glycolysis and gluconeogenesis fundamental biological pathways for energy conversion. Aldolase B enables fructose utilization by linking with enzymes like fructokinase and glucose-6-phosphatase during gluconeogenesis. Aldolase C participates in the glycolytic pathway associated with enzymes such as hexokinase supporting glucose breakdown during brain metabolism. Both enzymes maintain energy homeostasis within their respective tissues contributing to overall metabolic balance.
Aldolase B relates closely to hereditary fructose intolerance a genetic condition characterized by an inability to metabolize fructose properly in the liver. The deficiency or dysfunction of Aldolase B results in toxic accumulations impacting liver function. Aldolase C is associated with glioblastoma where its increased expression plays a role in tumor metabolism and growth. In the context of hereditary fructose intolerance Aldolase B is linked to fructokinase due to their sequential mechanisms in fructose metabolism while Aldolase C’s association in glioblastoma connects with altered metabolic pathways within cancerous cells.