Abcam, ab133357, Anti-CD11b antibody [EPR1344]

Size: 20µL / 100µL / 1mL
Anti-CD11b antibody [EPR1344] (ab133357) is a rabbit monoclonal antibody detecting CD11b in Western Blot, IHC-P, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 340 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1344,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, mIHC, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD11b antibody [EPR1344] (ab133357) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB and mIHC.
Anti-CD11b antibody [EPR1344] (ab133357) was first used in a scientific publication in 1976 and has been cited over 347 times in peer reviewed journals. It's performance in IHC in mouse and human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD11b antibody [EPR1344] (ab133357) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD11b antibody [EPR1344] (ab133357) has 31 independent reviews from customers.
Anti-CD11b antibody [EPR1344] (ab133357) specifically detects CD11b (UniProt ID: P11215; Molecular weight: 126kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR1344 -
ab209970
Antibody clone EPR1344 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor
488, Alexa Fluor
555, Alexa Fluor
647, Alexa Fluor
594, Alexa Fluor
568, Alexa Fluor
750, Biotin (
ab204472
ab307387
ab307391
ab307523
ab311811
ab313094
ab321047
ab322298
CD11b is involved in various diseases by regulating immune cell adhesion, migration and activation, which can influence inflammation, autoimmune conditions and cancer progression. Multiplex IHC staining using Opal in mouse spleen tissue
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary
CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.
Pathways
CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.
CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.