Abcam, ab133448, Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

Size: 20µL / 100µL / 1mL
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) is a rabbit monoclonal antibody detecting AMPK alpha 1 in Western Blot . Suitable for Drosophila melanogaster, Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 160 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5683,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Drosophila melanogaster,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.

Product details:
What is this antibody validated in?
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Drosophila melanogaster, Human, Mouse, Rat samples.
What is the molecular weight of AMPK alpha 1?
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) [EPR5683] (ab133448) specifically detects a band for AMPK alpha 1 (UniProt: Q13131) at a molecular weight of 64kDa.
Trusted by the scientific community
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) [EPR5683] (ab133448) was first used in a scientific publication in 2012 and has been cited over 160 times in peer-reviewed journals.
Reviewed by scientists
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) [EPR5683] (ab133448) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR5683] also available for your convenience: ab133448, Carrier free -
ab240058
, Carrier free -
ab271892
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle