Abcam, ab133473, Anti-Thiophosphate ester antibody [51-8]

Size: 50µg
Anti-Thiophosphate ester antibody [51-8] (ab133473) is a rabbit monoclonal antibody detecting Thiophosphate ester in IP . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:51-8,
Isotype:IgG,
Carrier free:No,
Applications:IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Specific to thiophosphorylated amino acids.

Product details:
What is this antibody validated in?
Anti-Thiophosphate ester antibody [51-8] (ab133473) is a rabbit recombinant monoclonal antibody and is validated for use in Immunoprecipitation (IP).
Trusted by the scientific community
Anti-Thiophosphate ester [51-8] (ab133473) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [51-8] also available for your convenience:
ab92570
, ab133473, Carrier free -
ab239919
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The thiophosphate ester often referred to as ATP gamma S is a structural analog of adenosine triphosphate (ATP) where the oxygen is substituted with sulfur at the gamma position. This modification results in resistance to hydrolysis making it useful for studying ATP-dependent processes. Although the exact mass of thiophosphate ester varies depending on the specific derivative it is widely utilized in various cellular and organ systems due to its involvement as an ATP analog. The compound is not expressible in living organisms but it is valuable in experimental designs in laboratory settings.
Biological function summary
The role of the thiophosphate ester centers around its use as a tool for modulating enzymatic activity in cellular processes. It often functions as part of complex biochemical assays to investigate phosphorylation mechanisms. As a gamma phosphate analog it non-hydrolyzably activates kinases and other ATP-binding proteins providing insights into their dynamics and interactions within cellular structures. Researchers commonly use it in studies involving protein phosphorylation and signal transduction.
Pathways
The thiophosphate ester plays a significant role in ATP-dependent phosphorylation pathways particularly those involving protein kinases. Its analog can mimic ATP in signal transduction pathways which leads to sustained activation of pathways like MAPK/ERK important for cell proliferation and differentiation. It shares relations with various kinases such as PKA and PKC which regulate diverse cellular responses. This serves to illustrate how its presence aids in understanding the regulation and execution of these pathways by inhibiting or prolonging their activity.
Thiophosphate ester analogs help researchers examine aberrant kinase signaling linked to cancer and neurodegenerative diseases. By stabilizing interactions with proteins such as p53 in cancer pathways it is possible to evaluate the role of faulty phosphorylation in tumorigenesis. In the context of neurodegenerative disorders the ester assists in investigating abnormal signal transduction pathways that may lead to conditions like Alzheimer’s disease. By providing a platform for studying protein interactions it contributes to the understanding of how improper regulation can lead to disease development and progression.