Abcam, ab133534, Anti-Rad51 antibody [EPR4030(3)]

Size: 100µL / 1mL
Anti-Rad51 antibody [EPR4030(3)] (ab133534) is a rabbit monoclonal antibody detecting Rad51 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 150 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4030(3),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IP, ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Rad51 antibody [EPR4030(3)] (ab133534) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB.
Anti-Rad51 antibody [EPR4030(3)] (ab133534) was first used in a scientific publication in 2013 and has been cited over 151 times in peer reviewed journals. It's performance in Western Blot and immunofluorescence in human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Rad51 antibody [EPR4030(3)] (ab133534) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Rad51 antibody [EPR4030(3)] (ab133534) has 13 independent reviews from customers.
Anti-Rad51 antibody [EPR4030(3)] (ab133534) specifically detects Rad51 (UniProt ID: Q06609; Molecular weight: 37kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR4030(3) -
ab221796
Antibody clone EPR4030(3) is also available pre-conjugated to a variety of labels for your convenience - PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
594, Alexa Fluor
555, Alexa Fluor
568, Alexa Fluor
ab303002
ab303003
ab303004
ab308698
ab309674
ab310037
ab310418
ab311945
ab312416
ab321050
Highly-cited antibody with >250 citations. A highly validated Rad51 antibody, essential for researchers studying DNA repair and homologous recombination. This antibody is crucial in genome stability research, particularly in understanding the DNA damage response. It is widely used in studies of Rad51 foci, cancer therapy and Rad51 inhibitors.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAD51 also known as recombination protein A or Novus RAD51 is a DNA repair protein with a molecular mass of approximately 37 kDa. This protein plays a significant role in homologous recombination and is active in the repair of double-strand breaks in DNA. It is expressed in various tissues with high levels seen in rapidly dividing cells. RAD51 acts by forming nucleoprotein filaments on single-stranded DNA and facilitates the search for homology and strand pairing during the repair process.
Biological function summary
Processes involving RAD51 are essential for genomic stability and cellular response to DNA damage. RAD51 often functions as part of a repair complex along with other proteins such as BRCA2 and RPA. This complex binds and stabilizes single-stranded DNA during search and strand invasion which is vital for successful recombination repair. Such repair mechanisms are important for preventing chromosomal aberrations and maintaining genetic integrity.
Pathways
RAD51's activity fits into the broader context of DNA damage repair pathways notably the homologous recombination (HR) pathway. This pathway is important for repairing double-strand breaks utilizing other proteins such as ATM and ATR to signal repair processes. Furthermore RAD51 interacts with various members of the BRCA protein family combining efforts to ensure proper DNA damage response and repair.
RAD51 mutations or dysregulation are linked to various types of cancer including breast and ovarian cancers. These cancers may involve mutations in BRCA1 and BRCA2 genes which affect RAD51's ability to effectively perform homologous recombination. Additionally disruptions in RAD51 function are associated with Fanconi anemia a disorder characterized by DNA repair defects. This condition demonstrates the importance of RAD51's interaction with FA pathway proteins in maintaining genomic stability and preventing disease.