Abcam, ab133744, Anti-ARIH2/TRIAD1 antibody [EPR7670]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ARIH2/TRIAD1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7670,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARIH2 also known as TRIAD1 is a RING-between-RING (RBR) E3 ubiquitin-protein ligase. It weights approximately 65 kDa. This protein facilitates the transfer of ubiquitin from E2 enzymes to substrates marking them for proteasomal degradation. ARIH2 is located mostly in the cytoplasm and nucleoplasm with high expression in immune cells such as macrophages and dendritic cells. It regulates ubiquitination which is important for controlling protein levels in the cell affecting cellular signal pathways and homeostasis.
Biological function summary
ARIH2 acts as an essential modulator of immune responses. By associating with the LUBAC complex (linear ubiquitin chain assembly complex) this protein contributes to the linear ubiquitination of specific substrates modulating immune signaling. Its activity affects the NF-κB signaling pathway a vital pathway in inflammation and immunity. ARIH2 influences anti-inflammatory responses and may balance immune homeostasis making it a critical player in immune system regulation.
Pathways
ARIH2 plays a significant role in the ubiquitylation pathway and the NF-κB signaling pathway. The protein cooperates with other members of the pathway like HOIP and SHARPIN to mediate immune responses through linear ubiquitin chains. This regulation influences the activation of NF-κB leading to changes in gene expression related to immune and inflammatory responses. ARIH2 is also involved in the regulation of the Wnt signaling pathway where it interacts with various signaling proteins to modulate developmental processes and cellular growth.
ARIH2 has implications in autoimmune diseases and cancer. Its regulation of immune responses connects it to autoimmune conditions where the body's immune system attacks its own cells. By influencing NF-κB signaling through proteins like LUBAC ARIH2 affects inflammation contributing to the pathogenesis of autoimmune diseases. In cancer ARIH2's role in protein degradation pathways can alter cell growth and survival connecting it to tumor development and progression. Its interaction with proteins such as HOIP in these pathways suggests its potential as a therapeutic target for both autoimmune disorders and cancer.