Abcam, ab134566, Anti-UPF3B/RENT3B antibody

Size: 100µg
Rabbit Polyclonal UPF3B/RENT3B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human UPF3B aa 300-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human UPF3B aa 300-350. The exact immunogen used to generate this antibody is proprietary information.Q9BZI7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UPF3B also known as RENT3B is a critical component of the nonsense-mediated mRNA decay (NMD) pathway which surveils and degrades mRNA transcripts with premature stop codons. UPF3B weighs approximately 54 kDa and primarily resides in the nucleus but can shuttle to the cytoplasm. Expression of UPF3B is notably prevalent in neuronal tissues suggesting a specialized role in brain function and development.
Biological function summary
UPF3B interacts with other proteins within the NMD complex to regulate mRNA stability and translation efficiency. As part of this complex UPF3B serves as a link between the nuclear and cytoplasmic phases of NMD. It interacts with UPF2 and UPF1 acting as a bridge to initiate downstream degradation processes. Through these interactions UPF3B balances gene expression and prevents the production of potentially harmful truncated proteins.
Pathways
UPF3B plays an important role in modulating the NMD pathway which is an important post-transcriptional regulatory mechanism in cells. UPF3B works alongside proteins like SMG1 and eIF4A3 within this pathway. It participates in the repression of aberrant mRNA and this function relates to RNA surveillance and cell cycle control. By ensuring faulty transcripts are removed UPF3B helps maintain cellular homeostasis and supports accurate gene expression profiles.
UPF3B mutations associate with X-linked intellectual disability (XLID) and schizophrenia. UPF3B's connection to XLID links it to the regulation of essential neurological functions possibly through its interaction with proteins like UPF2 and eIF4A3. These interactions indicate that proper functioning of NMD components including UPF3B is necessary for normal brain development and cognitive processes. Dysregulation or mutation of UPF3B can impact neural gene regulation leading to serious cognitive and mental health issues.