Abcam, ab134906, Anti-Gli1 antibody [EPR4523]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal GLI1 antibody. Suitable for WB and reacts with Human samples. Cited in 43 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4523,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Gli1 protein also known as GLI family zinc finger 1 acts mechanically as a transcription factor influencing gene expression. It comprises five C2H2-type zinc fingers enabling specific DNA binding activities. Gli1 has a molecular weight of approximately 118 kDa. Expression occurs prominently in skin brain and bone tissues. As an important effector in the Hedgehog (Hh) signaling pathway Gli1 transmits signals from the cell membrane to the nucleus.
Biological function summary
Gli1 functions to regulate cellular proliferation differentiation and survival. As part of the Gli protein family Gli1 operates in concert with other members such as Gli2 and Gli3 though it lacks significant repressor activity unlike some of its counterparts. It acts prominently in developmental processes controlling the expression of genes critical for embryogenesis. Gli1 also maintains stem cell populations and sustains tissue homeostasis in adults particularly in contexts where rapid cell division is necessary like wound healing.
Pathways
Gli1 plays a substantial role in the Hedgehog signaling pathway and interacts dynamically with this system. It receives upstream activation from the Smoothened protein which conveys signals in response to Hedgehog ligand availability. Additionally the Gli1 pathway overlaps with WNT signaling during various developmental stages. Through these interactions Gli1 coordinates tightly with other proteins to effect changes in target gene transcription that influence cell fate decisions.
Aberrant Gli1 activity associates strongly with certain cancers such as basal cell carcinoma and medulloblastoma. Persistent activation of the Hedgehog pathway often due to mutations in related proteins like Patched or Smoothened leads to excessive Gli1 activation and subsequent tumorigenesis. Beyond oncology Gli1's dysregulation also connects with congenital disorders such as Gorlin syndrome. Here mutations influence a broad network of genes that often include members of the Gli family causing developmental anomalies.