Abcam, ab135021, Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein

Size: 50µg
Recombinant human MLL + ASH2L + RBBP5 + WDR5 protein is a Human Fragment protein, in the 3745 to 3969 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Active,
Accession:P61964,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.4Preservative: 1.7% ImidazoleConstituents: 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.29% Tris HCl, 0.03% TCEP HCl, 0.00001% Zinc chloride

Product details:
50 μl reaction mix (50 mM Tris, pH 8.8, 5 mM MgCl
, 4 M DTT, 20 μM Sadenosylmethionine, and 5-50 ng MLL complex) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, incubate 30 minutes with the secondary HRP labeled antibody. Finally, add HRP chemiluminescent substrate and read luminescence.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MLL + ASH2L + RBBP5 + WDR5 complex also known as the SET1/COMPASS complex mechanically acts in gene regulation through histone modification. This multiprotein complex includes MLL (Myeloid-Lymphoid Leukemia) ASH2L (Absent Small or Homeotic 2-Like) RBBP5 (Retinoblastoma-Binding Protein 5) and WDR5 (WD Repeat Domain 5) with a combined mass that varies around 600 kDa. It expresses widely in different human tissues with notable activity in cells undergoing differentiation due to its essential role in chromatin remodeling and transcriptional activation.
Biological function summary
This complex plays an important role in the process of epigenetic regulation within cells. It functions by specifically catalyzing the methylation of histone H3 on lysine 4 (H3K4) which is associated with transcriptional activation. As a core component of the histone modification machinery the MLL complex significantly influences gene expression patterns. This activity regulates critical developmental and cellular differentiation processes highlighting its importance in maintaining healthy cell function.
Pathways
The MLL + ASH2L + RBBP5 + WDR5 complex integrates within the epigenetic signaling and transcriptional regulatory pathways. Its activity intersects with the Wnt signaling pathway which is essential for regulating embryonic development and cell proliferation. Additionally it interfaces with the Notch signaling pathway closely linked to cell differentiation processes. Proteins such as MEN1 (Menin 1) and others within the epigenetic network interact with this complex to exert their effects on downstream gene targets.
The aberrant function of the MLL + ASH2L + RBBP5 + WDR5 complex associates with mixed-lineage leukemia due to mutations or translocations involving MLL. This complex also relates to other malignancies as dysregulation of histone modification can contribute to oncogenesis. In particular mismanagement of the complex's activity can lead to poor outcomes in acute leukemia. It's connection to the protein DOT1L (Disruptor of Telomeric Silencing 1-Like) involved in another methyltransferase activity further emphasizes its relevance to diseases related to improper gene regulation.