Abcam, ab135249, Anti-PPM1A antibody [7F12]

Size: 100µL
Mouse Monoclonal PPM1A antibody. Suitable for Flow Cyt, ELISA, WB and reacts with Human, African green monkey, Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPM1A aa 200 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:7F12,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, African green monkey,
Applications:ELISA, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PPM1A aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P35813

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPM1A also known as protein phosphatase 1A or PP2C-alpha functions as a serine/threonine phosphatase. This protein plays an important role in removing phosphate groups from serine and threonine residues on proteins. The molecular mass of PPM1A is approximately 42-44 kDa. Expression of PPM1A is widespread and found in various human tissues indicating its significant involvement in multiple cellular processes.
Biological function summary
PPM1A regulates many cellular mechanisms by dephosphorylating targets and acting as a negative regulator of several signaling pathways. It modulates stress responses cell cycle progression and immune responses. PPM1A does not typically form complexes with other proteins but interacts closely with specific signaling molecules to control their activity. This regulation influences cellular homeostasis and growth in response to external and internal signals.
Pathways
PPM1A influences the TGF-beta signaling pathway where it interacts with and deactivates SMAD proteins by dephosphorylation. It also participates in the p38 MAPK pathway. These interactions highlight its role in controlling transduction signals initiated by growth factors and stress. By regulating these pathways PPM1A maintains a balance in cellular responses to growth and environmental stresses.
PPM1A links to cancer and inflammatory diseases. Its ability to modulate phosphorylation states impacts cell proliferation and immune responses critical factors in these conditions. For instance PPM1A interaction with SMAD3 in the TGF-beta pathway can influence tumor progression. Additionally its regulation of proteins such as p38 MAPK can alter inflammatory responses affecting inflammatory disease severity.