Abcam, ab135372, Anti-CD3 antibody [SP162]

Size: 20µL / 100µL / 500µL / 1mL
Anti-CD3 antibody [SP162] (ab135372) is a rabbit monoclonal antibody detecting CD3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP162,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD3 antibody [SP162] (ab135372) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Abcam's high quality manufacturing and validation processes ensure Anti-CD3 antibody [SP162] (ab135372) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD3 antibody [SP162] (ab135372) has 4 independent reviews from customers.
Anti-CD3 antibody [SP162] (ab135372) specifically detects CD3 (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone SP162 -
ab245731
Antibody clone SP162 is also available pre-conjugated to a variety of labels for your convenience - APC, HRP, Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
555, Alkaline Phosphatase (
ab305633
ab305634
ab307134
ab307265
ab307335
ab308972
Anti-CD3 antibody [SP162] (ab135372) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
CD3 is a crucial T cell co-receptor involved in activating both cytotoxic (CD8+) and helper (CD4+) T cells, playing a pivotal role in immune response. In immuno-oncology, targeting CD3 with therapeutic antibodies can enhance T cell-mediated anti-tumor activity, making it a valuable strategy in cancer treatment.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.6Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze