Abcam, ab136759, Rabbit F(ab')2 Anti-Dog IgG H&L (HRP)

Size: 500µL
Suitable for Flow Cyt, ELISA, IHC-P, WB, IHC-Fr, ICC. Ideal for western blot. Cited in 1 publication.
Key facts
Host species:Rabbit,
Target species:Dog,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:HRP,
Applications:ICC, ELISA, WB, IHC-P, IHC-Fr, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG

Product details:
Preparation of Substrate Solutions:
- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H
(30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H
. Reading at 492nm
- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H
(30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H
. Incubate 10 min at room temperature.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136759 is purified by affinity chromatography and is thus particularly specific., Storage buffer-Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C