Abcam, ab137973, Human Factor B ELISA Kit

Size: 1 x 96Tests
Human Factor B ELISA Kit is a sandwich ELISA used to quantify Human Factor B with a sensitivity of 0.97 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range – quantifies 2.2 – 140 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Milk, Plasma, Saliva, Serum, Cerebral Spinal Fluid,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:>= 0.97 ng/mL,
Range:2.188 - 140 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human Factor B ELISA Kit ab137973 is a sandwich ELISA kit to measure Human Factor B in Serum, Plasma, Milk, Saliva, and Cerebral Spinal Fluid with a sensitivity of 0.98 ng/mL.
How the assay works
Human Factor B ELISA Kit ab137973 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay Specificity
This kit recognizes both native human Factor B in serum, plasma, saliva, milk and CSF samples. No significant cross-reactivity observed with Human Complement factor D, Factor H, Factor I, Factor P, C1, C2, C3, C4, C5, C6, C7,C8, and C9 proteins.
Human Factor B ELISA Kit ab137973 protocol summary:
1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
How other researchers are using Human Factor B ELISA Kit ab137973
Human Factor B ELISA Kit ab137973 has been used to study complement activation in acute respiratory disease
, the progression of renal cancer
, and alternative complement pathway activation in severe dengue
References:
W. Bain et al., 2023, PMID: 37377659
L. Cooley et al., 2021, PMID: 34670568
S. Cabezas-Falcon et al., 2021, PMID: 33410734,
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
Biological function summary
Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.
Pathways
Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.
Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.