Abcam, ab137987, Human Protein C ELISA Kit

Size: 1 x 96Tests
Human Protein C ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Protein C in Human in Cell/Tissue Extracts, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Cell Lysate, Saliva, Urine, Milk,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 ng/mL,
Range:12.5 - 100 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Protein C Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein C in urine, saliva, milk, cell culture supernatant, cell lysate and tissue samples.
A Protein C specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein C specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein C captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein C is a vitamin K-dependent serine protease enzyme that plays an important role in blood coagulation. It is also known as autoprothrombin IIA or blood coagulation factor XIV. The molecular weight of Protein C is approximately 62 kDa. It is synthesized in the liver and circulates in the plasma as an inactive zymogen. The protein becomes activated on the surface of endothelial cells and platelets where it plays an anticoagulant role.
Biological function summary
Protein C serves as an important regulator in the coagulation pathway. Upon activation it joins with a cofactor known as Protein S to form the activated Protein C complex which inactivates clotting factors Va and VIIIa. This inactivation leads to a reduction in thrombin formation thereby preventing excessive blood clotting. The activated C complex works efficiently on phospholipid surfaces such as those found on cellular membranes.
Pathways
Protein C activation is an important step in the anticoagulant pathway and works to balance the procoagulant actions of other factors. Protein C functions in tandem with proteins such as thrombin which transforms it into its active form when bound to thrombomodulin on the endothelial cell surface. The activated Protein C pathway ensures proper blood flow by preventing unchecked clot propagation and is intricately tied with pathways involving thrombin and factors Va and VIIIa.
Protein C deficiency is associated with an increased risk of developing thromboembolic disorders like deep vein thrombosis and pulmonary embolism. Deficiency can be congenital or acquired resulting in uncontrolled coagulation and increased susceptibility to clot formation. Additionally impaired activity of protein C is related to a disorder known as purpura fulminans which is characterized by blood clot formation and skin necrosis. The relation of Protein C to these conditions often involves dysfunctional interactions with factors Va VIIIa and thrombin.