Abcam, ab137994, Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT)

Size: 1 x 96Tests
Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) is a sandwich ELISA designed to quantify Mouse Thrombin-Antithrombin Complexes (TAT) with a sensitivity of 1.5 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 1.953 - 500 pg/mL - Cited in over 20 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:>= 1.5 pg/mL,
Range:1.953 - 500 pg/mL,
Assay time:5h,
Assay Platform:Microplate

Product details:
Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) ab137994 is a sandwich ELISA to measure Mouse Thrombin-Antithrombin Complexes (TAT) in plasma with a sensitivity of 1.5 pg/ml.
How the assay works
A TAT Complexes specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TAT Complexes specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TAT Complexes captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) ab137994 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Thrombin-Antithrombin Complex also referred to as TAT complex plays a significant mechanical role in regulating blood coagulation. It forms from the interaction of thrombin an enzyme and antithrombin a serpin inhibitor. Thrombin shows an approximate molecular mass of 36 kDa while antithrombin weighs around 58 kDa. This complex is expressed in the liver and circulates in the bloodstream where it helps maintain hemostasis by neutralizing excess thrombin preventing unwanted clotting events.
Biological function summary
The complex participates in the regulation of the coagulation cascade. It functions as an essential inhibitor forming part of a larger set of interactions that ensure blood coagulation occurs correctly. As a result it plays a role in maintaining the balance between coagulation and anticoagulation reducing the risk of thrombotic conditions. The TAT complex can be detected using specific kits like TAT kits which measure its levels important for understanding coagulation status in conditions using both human and mouse models.
Pathways
The thrombin-antithrombin complex is integrally involved in the intrinsic and extrinsic pathways of the coagulation cascade. It serves as a feedback mechanism to control and limit thrombin activity. The balance it provides is important to the effective regulation of fibrin formation working alongside proteins such as fibrinogen and factor Xa. Antithrombin in particular requires heparin or heparin-like molecules to enhance its inhibitory effects on thrombin ensuring precise modulation of coagulation.
Thrombin-antithrombin complex levels correlate with various thrombotic and hemorrhagic disorders. Elevated levels often indicate ongoing activation of the coagulation system seen in conditions like deep vein thrombosis and disseminated intravascular coagulation (DIC). These elevated levels can result from increased thrombin generation and antithrombin consumption. The complex's interaction with proteins like factor V and fibrin is pertinent in the pathology of these disorders making it a valuable marker in diagnosis and monitoring therapeutic responses.