Abcam, ab138492, Anti-Collagen I antibody [EPR7785]

Size: 100µL / 1mL
Anti-Collagen I antibody [EPR7785] (ab138492) is a rabbit monoclonal antibody detecting Collagen I in Western Blot, IHC-P, IHC-Fr, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 250 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7785,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Compared with ab138492, ab255809 has higher affinity. We recommend ab255809 as an alternative for testing pro-Collagen forms in western blot. ab138492 works in western blot in samples with high level of collagen I, like HFF-1, MRC-5, skin tissue etc.ab138492 is specific for pro-Collagen and 139kda mature from, while ab255809 is specific for pro-Collagen and 35kda C-terminal pro peptide.Please navigate to the Product Protocol section for specificity details in Chinese.

Product details:
Anti-Collagen I antibody [EPR7785] (ab138492) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-Fr IHC-P and WB.
Anti-Collagen I antibody [EPR7785] (ab138492) was first used in a scientific publication in 2014 and has been cited over 253 times in peer reviewed journals. It's performance in Western Blot and IHC in human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Collagen I antibody [EPR7785] (ab138492) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Collagen I antibody [EPR7785] (ab138492) has been confirmed by testing in Collagen I knockout cells.
Anti-Collagen I antibody [EPR7785] (ab138492) has 34 independent reviews from customers.
Anti-Collagen I antibody [EPR7785] (ab138492) specifically detects Collagen I (UniProt ID: P02452; Molecular weight: 95kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR7785 -
ab215969
Antibody clone EPR7785 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, Alexa Fluor
594, Alexa Fluor
568, Alexa Fluor
555, Alexa Fluor
ab275996
ab280968
ab311739
ab313018
ab313221
ab321114
Collagen I modulates the tumor microenvironment by enhancing tissue rigidity and facilitating neoplastic cell proliferation. Additionally, it contributes to immune evasion by forming a structural barrier that impedes T-cell infiltration and activity.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.