Abcam, ab139216, Recombinant Human PGPEP1 protein

Size: 100µg
Recombinant Human PGPEP1 protein is a Human Full Length protein, in the 1 to 209 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9NXJ5,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PGPEP1 also known as pyroglutamyl-peptidase I is an enzyme responsible for the removal of pyroglutamyl (pGlu) groups from the N-terminus of peptides and proteins an important step for protein regulation and maturation. The enzyme typically shows broad substrate specificity. PGPEP1 has an approximate mass of 23 kDa and is expressed in various tissues although its expression levels differ depending on the tissue type. Researchers often examine its expression in the liver and kidney where it is significantly active.
Biological function summary
The removal of the pGlu group by PGPEP1 modifies peptide stability and activity impacting processes like metabolism and signal transduction. PGPEP1 operates as a monomer and does not form part of larger protein complexes. By regulating the degradation of pyroglutamylated proteins it plays an important role in maintaining protein homeostasis within cells.
Pathways
PGPEP1 influences pathways such as protein catabolism and peptide hormone processing where the removal of pGlu groups aids in maintaining proteolytic activity and modulating bioactive peptides. Its activity intersects with hormones like thyrotropin-releasing hormone (TRH) which it processes. PGPEP1 also interacts with other enzymes like aminopeptidases involved in sequential processing of peptides after the initial pyroglutamyl removal.
Research indicates a connection between abnormal PGPEP1 activity and metabolic syndromes such as obesity due to its role in hormonal regulation and peptide processing. Studies also link PGPEP1 to cognitive disorders where it affects neuropeptide processing in neural tissues. Its interaction with hormones like TRH highlights a potential relationship with proteins such as thyroxine-binding globulin in managing these conditions.