Abcam, ab139365, Anti-MHC Class II antibody [M5/114]

Size: 100µg
Anti-MHC Class II antibody [M5/114] (ab139365) is a rat monoclonal antibody detecting MHC Class II in Western Blot, Flow Cytometry, IP, FuncS, BL . Suitable for Mouse .
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:M5/114,
Isotype:IgG2b,
Carrier free:Yes,
Reacts with:Mouse,
Applications:WB, FuncS (Neut/Block), Flow Cyt, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing H2-Ea protein. The exact immunogen used to generate this antibody is proprietary information.P01904,
Specificity:ab139365 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. ab139365 can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Product details:
What is this antibody validated in?
Anti-MHC Class II antibody [M5/114] (ab139365) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Functional Studies, Blocking in Mouse samples.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity is > 95% by SDS-PAGE and 0.2 μm filter sterilized., Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHC Class II also known as MHC II or human leukocyte antigen (HLA) class II is a protein complex with a molecular mass generally around 55 kDa. This protein is mainly expressed on the surface of antigen-presenting cells such as dendritic cells macrophages and B cells. It plays an important role in the immune system by presenting extracellular antigens to T helper cells. MHC II molecules are composed of two polypeptide chains alpha and beta which associate to form a heterodimer on the cell surface.
Biological function summary
MHC Class II molecules facilitate the immune system's recognition and response to pathogens by presenting processed antigenic peptides to CD4+ T lymphocytes. This activity involves their assembly within the endosomal-lysosomal system where they bind peptides derived from endocytosed proteins. MHC II is part of the major histocompatibility complex which includes other related molecules such as MHC Class I (HLA class I). The binding and presentation of peptides are central for the coordination of adaptive immune responses enhancing pathogen clearance and maintaining immunological memory.
Pathways
MHC Class II operates within the antigen processing and presentation pathway important for adaptive immunity. Its interaction with CD4+ T cells stimulates downstream signaling pathways leading to T cell activation and differentiation. Related proteins in the antigen presentation pathway include invariant chain (Ii) which guides MHC II to the endosome and HLA-DM which facilitates peptide loading. Together these proteins and pathways work to ensure effective immune surveillance and response.
Alterations in MHC Class II expression or function are associated with autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. In these conditions improper antigen presentation may lead to an inappropriate immune response against self-proteins. The involvement of MHC II in disease is often linked to specific HLA alleles which vary among individuals and influence susceptibility to autoimmune disorders. Additionally the abnormal function of connected proteins like cytokines from activated T helper cells can exacerbate these immune conditions.