Abcam, ab139403, Anti-alpha 1 Spectrin antibody [EPR9300]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal alpha 1 Spectrin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9300,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha 1 Spectrin also known as SPTA1 is an essential protein that plays a mechanical role in cells. This protein has a large molecular mass of approximately 280 kDa and is predominantly expressed in erythrocytes but it is also found in other cell types. Structurally it forms part of the spectrin family which includes alpha and beta spectrin subunits. Alpha 1 Spectrin interacts with the actin cytoskeleton creating a network that supports the plasma membrane’s integrity and flexibility.
Biological function summary
Alpha 1 Spectrin binds with other proteins to form a spectrin-actin complex that is essential for maintaining cell shape and mechanical stability. It interacts with ankyrin and other components of the cytoskeleton to provide the necessary elasticity and durability to cells especially red blood cells. This protein makes sure that the cytoskeletal network remains stable and efficiently responds to mechanical stresses from the outside environment.
Pathways
Alpha 1 Spectrin is involved in critical pathways important for cellular functions. It participates in the erythrocyte cytoskeleton organization pathway interacting with proteins such as ankyrin and protein 4.1R. These interactions influence cell deformability and help maintain the biconcave shape of red blood cells allowing them to navigate through narrow capillaries effectively. Alpha 1 Spectrin’s functionality ensures efficient oxygen delivery throughout the body by maintaining red blood cell integrity.
Mutations or deficiencies in Alpha 1 Spectrin can lead to conditions such as hereditary spherocytosis and elliptocytosis. These disorders are characterized by hemolytic anemia and are linked to disruptions in the cytoskeleton structure and function of erythrocytes. In these cases Alpha 1 Spectrin can display abnormal interactions with related proteins like ankyrin leading to compromised membrane stability and shortened red blood cell lifespan. The understanding of Alpha 1 Spectrin’s role in these diseases provides insight into potential therapeutic targets for managing these disorders.