Abcam, ab139686, Anti-ME2 antibody [EP7217]

Size: 100µL
Rabbit Monoclonal ME2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 10 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP7217,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein ME2 also known as mitochondrial malic enzyme 2 is a mitochondrial enzyme with a molecular mass of approximately 65 kDa. It catalyzes the oxidative decarboxylation of malate to pyruvate in the tricarboxylic acid cycle converting NAD+ to NADH. ME2 is primarily expressed in tissues with high metabolic activity such as liver and muscle. The enzyme plays a role in energy production and is found across various species reflecting its evolutionary conservation.
Biological function summary
ME2 is an important regulator of cellular metabolism and part of the mitochondrial malate-aspartate shuttle. The enzyme helps facilitate the transfer of reducing equivalents across the mitochondrial membrane. ME2 does not function alone and might interact with other metabolite transporters in mitochondrial membranes contributing to the balance of metabolic intermediates and maintenance of cellular energy homeostasis.
Pathways
Malate metabolism and energy production involve ME2's role in the tricarboxylic acid cycle and glycolysis. The tricarboxylic acid cycle requires ME2 for effective conversion of nutrients into usable energy. ME2 links with other metabolic enzymes including citrate synthase for efficient energy flow. Additionally the enzyme's activity affects the synthesis of biosynthetic intermediates integrating nutrient signals with bioenergetic demands.
ME2 alterations can impact conditions like metabolic syndromes and neurodegenerative diseases. Research indicates ME2 changes might relate to diabetes where altered energy metabolism disrupts insulin signaling. ME2 also bears connection to Alzheimer's disease likely due to its involvement in mitochondrial dysfunction a characteristic of the disorder. Understanding these links can guide therapeutic strategies aimed at correcting metabolic disturbances.