Abcam, ab140175, Anti-FBXL5 antibody

Size: 100µg
Rabbit Polyclonal FBXL5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXL5 aa 500 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FBXL5 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UKA1

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute in 200 µL of water, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FBXL5 also known as F-box and leucine-rich repeat protein 5 is a part of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This protein weighs approximately 82 kDa and plays a direct role in ubiquitination and subsequent proteasomal degradation of specific substrate proteins. It is expressed across various tissues emphasizing the liver and brain indicating its potential contribution to systemic regulatory roles.
Biological function summary
FBXL5 regulates cellular iron homeostasis by targeting iron regulatory proteins particularly IRP2 for degradation. FBXL5 operates within a complex environment and importantly serves as a sensor for both iron and oxygen levels influencing the translation of mRNAs coding for proteins involved in iron metabolism. It maintains cellular iron balance preventing the excess or deficiency that can lead to cellular dysfunction or stress.
Pathways
FBXL5 integrates into the iron metabolism pathway and is vital for the iron-sulfur cluster assembly pathway as well. Its interactions with other proteins such as IRP1 and IRP2 mediate the expression of genes responsible for iron uptake utilization and storage. FBXL5's regulatory influence includes key proteins like transferrin receptor and ferritin directly affecting iron distribution.
FBXL5 dysfunction has associations with iron metabolism disorders such as anemia and neurodegenerative diseases like Parkinson's disease. Alterations in FBXL5 levels or activity impact iron accumulation which in turn induces oxidative stress and cellular damage both of which are linked to these diseases. The protein's relationship with IRP2 signifies its involvement in maintaining iron homeostasis and its potential contribution to disease progression when dysregulated.