Abcam, ab140416, Recombinant Human SAE2 / UBA2 protein

Size: 20µg
Recombinant Human SAE2 / UBA2 protein is a Human Full Length protein, in the 1 to 640 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB.
Key facts
Purity:>85% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9UBT2,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SAE2 also known as UBA2 is a protein involved mechanically in the SUMO (Small Ubiquitin-like Modifier) conjugation pathway. It functions as an E1 activating enzyme collaborating closely with its partner SAE1 in the initiation of SUMOylation. SAE2 has a known molecular mass of approximately 72 kDa. It is widely expressed in various tissues with high expressions noted in the nucleus. This expression pattern suggests it plays a role in maintaining cellular processes under a variety of conditions.
Biological function summary
This protein plays an important role in the post-translational modification process of SUMOylation which is critical for regulating protein function. SAE2 is part of the E1 activating enzyme complex working specifically with SAE1 to activate and transfer SUMO to target proteins. It contributes to cellular processes such as nuclear-cytosolic transport chromosome segregation and DNA repair. This indicates its functions are central to maintaining genomic stability and cellular homeostasis.
Pathways
SAE2 is integral in the SUMOylation pathway which interacts with critical cellular pathways like the DNA damage response and cell cycle regulation pathways. It has important interactions with proteins such as UBE2I (also known as Ubc9) the only E2 enzyme in the SUMOylation cascade and PIAS family proteins which function as E3 ligases. These connections allow SAE2 to influence various pathways essential for cell survival and proliferation.
SAE2 influences pathological conditions such as cancer and neurodegenerative diseases. Its role in SUMOylation connects it to the regulation of oncogenic pathways potentially linking it with proteins like p53 a well-known tumor suppressor. In neurodegenerative diseases alterations in SUMOylation pathways with SAE2 as a participant might affect proteins involved in neuron function and survival contributing to disease progression. Understanding SAE2's interactions in these diseases might provide insights into therapeutic strategies.