Abcam, ab140722, Recombinant Human TDP2 protein

Size: 50µg
Recombinant Human TDP2 protein is a Human Full Length protein, in the 1 to 362 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O95551,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TDP2 also known as Tyrosyl-DNA phosphodiesterase 2 functions mechanically by repairing DNA through removing topoisomerase II-DNA adducts. It has a mass of approximately 53 kDa. TDP2 shows expression in various tissues with notable levels in brain and muscle. The enzyme exhibits phosphodiesterase activity which is essential for resolving stalled topoisomerase-2-mediated breaks during the repair process.
Biological function summary
TDP2 plays an important role in DNA repair mechanisms. It participates in excising 5'-phosphotyrosyl moieties linked to DNAn important for maintaining genomic stability. TDP2 does not work as a part of a large protein complex but its activity supports several cellular processes by ensuring proper DNA repair. It facilitates the release of covalent protein-DNA complexes therefore aiding cell survival and function.
Pathways
TDP2 contributes to the DNA damage response and repair pathways. It acts in concert with proteins like XRCC1 and PARP1 to ensure repair of DNA strand breaks. Additionally TDP2 is involved in the non-homologous end joining (NHEJ) pathway an important mechanism for repairing double-strand breaks. Its function complements topoisomerase-related activities maintaining genomic integrity during cell division.
TDP2 has been connected to neurodegenerative diseases and certain cancers. Mutations or dysfunctions in TDP2 can lead to spinocerebellar ataxia and intellectual disability as the enzyme’s role in DNA repair is compromised. In cancer disruptions in TDP2 activity can influence cancer progression with interactions involving proteins like ATM playing a role in oncogenesis. Understanding these relationships highlights potential therapeutic targets for treatment strategies.