Abcam, ab145248, DAN, Fluorescence determination of NO2-

Size: 1g / 5g
MW 158.2 g/mol, Purity >98%. Routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay uses the reaction of DAN with NO 2 - under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a derivatisation-reagent for the determination of selenium at low detection limits.
Key facts
CAS number:771-97-1,
Purity:>98%,
Form:SolidSee storage information,
Molecular weight:158.2 g/mol,
Nature:Synthetic,
Solubility:Soluble in DMSO,
Biological description:Routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a derivatisation-reagent for the determination of selenium at low detection limits.,
InChi:InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2,
InChiKey:XTBLDMQMUSHDEN-UHFFFAOYSA-N,
IUPAC Name:2,3-Diaminonaphthalene,
Excitation/Emission:Ex: 364nm, Em: 406nm

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-The product can be stored for up to 12 months