Abcam, ab147053, WIKI4, Wnt/ beta catenin inhibitor

Size: 5mg / 25mg
MW 521.6 Da, Purity >98%. WIKI-4 is a potent inhibitor of Wnt/β-catenin signaling EC 50 ~ 75 nM (in all cell lines tested including DLD1 colorectal cancer cells, NALM6 B cells, U-2 OS osteosarcoma cells, and hESCs); inhibits auto-ADP-ribosylation of tankyrase 2 (TNKS2) (IC 50 ~15 nM). Also a potent and selective inhibitor of TNKS1 and TNKS2 (IC 50 s = 26 and 15 nM, respectively). It has little or no effect on related polymerases or transferases at 10 μM. Through its effects on TNKS2, WIKI4 prevents AXIN ubiquitinylation and degradation, blocking signaling through the Wnt/β-​catenin pathway. WIKI4 inhibits the expression of β-​catenin target genes and cellular responses to Wnt/β-​catenin signaling in both cancer cell lines and embryonic stem cells.
Key facts
CAS number:838818-26-1,
Purity:>98%,
Form:SolidSee storage information,
Molecular weight:521.6 Da,
Molecular formula:C29H23N5O3S,
PubChem:2984337,
Nature:Synthetic,
Solubility:Soluble in DMSO to 20 mM,
Biochemical name:2-[3-[[4-(4-Methoxyphenyl)-5-pyridin-4-yl-1,2,4-triazol-3-yl]sulfanyl]propyl]benzo[de]isoquinoline-1,3-dione,
Biological description:WIKI-4 is a potent inhibitor of Wnt/β-catenin signaling EC50 ~ 75 nM (in all cell lines tested including DLD1 colorectal cancer cells, NALM6 B cells, U-2 OS osteosarcoma cells, and hESCs); inhibits auto-ADP-ribosylation of tankyrase 2 (TNKS2) (IC50 ~15 nM). Also a potent and selective inhibitor of TNKS1 and TNKS2 (IC50s = 26 and 15 nM, respectively). It has little or no effect on related polymerases or transferases at 10 μM. Through its effects on TNKS2, WIKI4 prevents AXIN ubiquitinylation and degradation, blocking signaling through the Wnt/β-​catenin pathway. WIKI4 inhibits the expression of β-​catenin target genes and cellular responses to Wnt/β-​catenin signaling in both cancer cell lines and embryonic stem cells,
Canonical smiles:COC1=CC=C(C=C1)N2C(=NN=C2SCCCN3C(=O)C4=CC=CC5=C4C(=CC=C5)C3=O)C6=CC=NC=C6,
InChi:InChI=1S/C29H23N5O3S/c1-37-22-11-9-21(10-12-22)34-26(20-13-15-30-16-14-20)31-32-29(34)38-18-4-17-33-27(35)23-7-2-5-19-6-3-8-24(25(19)23)28(33)36/h2-3,5-16H,4,17-18H2,1H3,
InChiKey:RNUXIZKXJOGYQP-UHFFFAOYSA-N,
IUPAC Name:2-[3-[[4-(4-methoxyphenyl)-5-pyridin-4-yl-1,2,4-triazol-3-yl]sulfanyl]propyl]benzo[de]isoquinoline-1,3-dione

Product details:
This product is manufactured by BioVision, an Abcam company and was previously called B1630 WIKI-4. B1630-25 is the same size as the 25 mg size of ab147053.
Protect from air and light.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Store in the dark, Store under desiccating conditions