Abcam, ab14748, Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

Size: 100µg
Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) is a mouse monoclonal antibody detecting ATP5A in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Cow, Drosophila melanogaster, Human, Mouse, Rat . - Clone 15H4C4 is the most cited clone to ATP5A - Over 550 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:15H4C4,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human, Drosophila melanogaster,
Applications:WB, IHC-P, Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) has been cited over 553 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) has high sensitivity and specificity.
Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) has 25 independent reviews from customers.
Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) specifically detects ATP5A (UniProt ID: P25705; Molecular weight: 55kDa) and is sold in 100 µg selling sizes.
Antibody clone 15H4C4 is also available pre-conjugated to FITC for your convenience (
ab119688
ATP5A (ATP Synthase F1 Subunit Alpha) is an essential protein for ATP production in mitochondria, forming part of the ATP synthase complex. This complex is crucial for oxidative phosphorylation, the process by which cells generate energy. Mutations in the ATP5A gene are linked to mitochondrial disorders, including combined oxidative phosphorylation deficiency 22 (COXPD22), which manifests as severe metabolic and neurological issues, emphasizing the protein's critical role in energy metabolism.
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Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.
Biological function summary
ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.
Pathways
ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.
ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson’s disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.