Abcam, ab14993, Anti-PEDF antibody

Size: 100µg
Rabbit Polyclonal PEDF antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human SERPINF1.P36955,
Specificity:Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms.

Product details:
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: 30% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.184% Tris glycine, Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C, Aliquoting information-Add glycerol to a final volume of 50% for extra stability and aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.
Biological function summary
PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.
Pathways
PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.
PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.