Abcam, ab15085, Anti-Polyoma virus, Medium T antigen antibody [PyMT]

Size: 100µg
Rat Monoclonal MT antibody. Suitable for IP, WB, ELISA, ICC/IF and reacts with Polyomavirus, Synthetic peptide - Polyomavirus samples. Cited in 21 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:PyMT,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Polyomavirus,
Applications:ICC/IF, IP, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Binds medium T antigen only, allows isolation of viral T antigens.

Properties and Storage Information:
Form-Liquid, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Polyoma virus Medium T antigen also called Middle T antigen belongs to the early proteins expressed by Polyomaviruses. Medium T antigen has a molecular mass of about 57 kilodaltons (kDa). This protein is located in the cytoplasm and is anchored to the cellular membrane. Its expression occurs predominantly during the early phase of viral infection. The Medium T antigen has an important role in transforming host cells primarily through its interactions with cellular signaling components.
Biological function summary
The Middle T antigen facilitates oncogenic transformation by associating with host cell proteins that regulate various signaling pathways. This protein engages in forming a complex with cellular kinases such as Src family kinases. By acting as a scaffold protein it effectively recruits and activates these kinases which contributes to unregulated cell growth. Through these interactions Medium T antigen alters normal cell signaling dynamics and promotes cellular transformation an important step in viral proliferation.
Pathways
The Middle T antigen integrates into signaling networks that control cell growth and division. It prominently interfaces with the PI3K/Akt pathway which is essential for cell proliferation and survival. In this context the Medium T antigen interacts closely with Protein Tyrosine Kinase-driven pathways including the Src kinase pathway influencing downstream targets. These interactions facilitate abnormal activation of signaling projects linked to cancer development.
The Middle T antigen's role links closely to cancer formation specifically in relation to its ability to induce tumorigenesis. Its interactions with proteins like the Src family kinases can lead to uncontrolled cellular proliferation and transformation phenomena observed in cancerous conditions. The aberrant engagement of Medium T antigen with PI3K further emphasizes its connection to cancer as dysregulated PI3K/Akt signaling is common in various malignancies.