Abcam, ab151754, Anti-ALAD antibody [EPR10268(B)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal ALAD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10268(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ALAD also known as Aminolevulinate Dehydratase is an essential enzyme involved in the biosynthesis of heme facilitating the condensation of two molecules of aminolevulinic acid to form porphobilinogen. The enzyme has a molecular mass of approximately 36 kDa. It is ubiquitously expressed across various tissues with notable activity in the liver and erythroid precursor cells within the bone marrow where heme biosynthesis is essential for the production of hemoglobin.
Biological function summary
The enzyme plays an important role in the early steps of the heme biosynthetic pathway. ALAD forms part of a multi-step process critical for heme production which is an important component of hemoglobin myoglobin and various cytochromes. The enzyme operates as a metalloenzyme requiring zinc for its activity and is subject to regulation by metal ion availability and substrate concentrations. Alterations in its activity can have significant impacts on cellular iron utilization and red blood cell function.
Pathways
ALAD plays a critical role within the heme biosynthesis pathway linking amino acid metabolism to porphyrin and heme production. It connects tightly with other enzymes involved in this pathway such as porphobilinogen deaminase. ALAD's function ensures the steady supply of intermediates necessary for synthesizing heme which serves further purposes in electron transport and oxygen transport systems within cells.
The improper function of ALAD associates with conditions such as lead poisoning and acute intermittent porphyria. In lead poisoning lead ions inhibit ALAD activity resulting in the accumulation of its substrate aminolevulinic acid and subsequent disturbances in heme production. In acute intermittent porphyria mutations in ALAD or interacting proteins like porphobilinogen deaminase can influence enzyme efficiency and increase the risk of metabolic crises. Understanding ALAD's role offers potential therapeutic insights for managing these disorders.