Abcam, ab15260, Anti-14-3-3 beta antibody

Size: 500µL / 1mL
Rabbit Polyclonal 1433B phospho S184 + S186 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.6Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
14-3-3 beta also known as YWHAB is a protein that electrically neutralizes and stabilizes partner proteins. It has a mass of about 30 kDa and is part of the highly conserved 14-3-3 protein family. This family is ubiquitous in eukaryotic cells with 14-3-3 beta being expressed in many tissues including the brain and muscles. Its mechanical role involves binding phosphorylated serine and threonine residues which influences the conformation and function of the target protein.
Biological function summary
14-3-3 beta plays a central role in cellular processes like signal transduction apoptosis and cell cycle control. It often acts in concert with other proteins forming complexes that regulate these vital functions. For instance it binds to Raf-1 kinase affecting cell growth and differentiation. It also interacts with proteins involved in the mitogen-activated protein kinase (MAPK) pathway highlighting its diverse biological significance.
Pathways
14-3-3 beta is important in both the MAPK and apoptotic pathways. In the MAPK pathway it helps regulate the phosphorylation status of proteins involved in cell proliferation. The protein binds to Raf-1 an important component in MAPK signaling impacting cellular responses to growth signals. In the context of apoptosis 14-3-3 beta partners with BAD protein a pro-apoptotic factor sequestering it away from mitochondria this interaction is critical for cell survival by preventing the apoptotic process.
Disruptions in 14-3-3 beta function associate with neurodegenerative diseases and cancer. For example its interaction with proteins like Tau connected to Alzheimer's disease points to its involvement in neurodegeneration. Aberrations in its regulatory roles can also contribute to uncontrolled cell growth linked to cancer. Furthermore its interaction with Bcl-2 family proteins ties it to variations in apoptotic regulation seen in cancerous cells.