Abcam, ab153254, Recombinant Human SAMHD1 protein

Size: 10µg
Recombinant Human SAMHD1 protein is a Human Full Length protein, in the 1 to 626 aa range, expressed in Wheat germ, with >80%, suitable for ELISA, WB.
Key facts
Purity:>80%,
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9Y3Z3,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SAMHD1 also known as Sterile Alpha Motif and HD-Domain Containing Protein 1 functions mechanically as a dGTP-dependent deoxynucleotide triphosphohydrolase. It has a molecular mass of approximately 72 kDa. This protein is expressed in various cell types most notably in immune cells such as macrophages and dendritic cells. SAMHD1 operates by hydrolyzing deoxynucleoside triphosphates (dNTPs) into deoxynucleosides and inorganic triphosphates controlling the intracellular pool of dNTPs.
Biological function summary
SAMHD1 serves as a modulator of cellular proliferation and DNA repair processes. It forms part of a larger complex that regulates DNA replication through its influence on dNTP levels. By modulating these levels the protein prevents excess dNTP buildup which can result in inefficient replication and genomic instability. Moreover SAMHD1 contributes to the innate immune response by restricting viral replication particularly that of retroviruses like HIV-1 in non-dividing cells.
Pathways
SAMHD1 participates in nucleotide metabolism and DNA damage response pathways. For effective dNTP pool regulation it interacts with proteins involved in dNTP synthesis and degradation such as RRM2B. Additionally SAMHD1 is relevant in pathways connected to the restriction of viral infections working alongside proteins like TREX1 that degrade excess DNA and sustain genomic integrity during infection.
SAMHD1 mutations associate with autoimmune disorders and certain types of cancers. Aicardi-Goutières syndrome a rare genetic inflammatory disorder shows links to defective SAMHD1 activity where impaired DNA damage response leads to chronic inflammation. Furthermore altered SAMHD1 expression relates to chronic lymphocytic leukemia where it influences both the proliferation and survival of malignant cells. Interaction with proteins such as DNA-PK is significant in linking SAMHD1 to these conditions demonstrating its role in maintaining immune system balance and genomic stability.