Abcam, ab153787, Recombinant Human Nectin 3 protein

Size: 10µg
Recombinant Human Nectin 3 protein is a Human Fragment protein, in the 58 to 366 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:HPLC, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9NQS3,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in PBS,
Storage buffer:pH: 7.4Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

Product details:
Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-A few weeks, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nectin-3 also known as CD113 or PVRL3 is an immunoglobulin-like cell adhesion molecule. It plays an important role in cell-cell adhesion by forming homo- and heterophilic trans-dimers with other nectins and nectin-like molecules. Nectin-3 has a molecular mass of approximately 83 kDa. Its expression is generally observed in neural tissues such as the brain as well as in testis and various epithelial tissues. This distribution allows it to contribute to diverse cellular interactions critical for tissue organization and development.
Biological function summary
Nectin-3 participates in the formation and maintenance of adherens junctions by interacting with cadherins and actin filaments. It is a member of the Nectin family which includes Nectin-1 Nectin-2 and Nectin-4 known for their roles in cell adhesion. The function of Nectin-3 is integral to the establishment of tight junctions contributing to the structural integrity and function of epithelial and endothelial cells. It often works as part of a complex with afadin which supports its connection to the actin cytoskeleton.
Pathways
Nectin-3 acts in the formation of synapses and is involved in signaling pathways related to cell adhesion such as the actin cytoskeleton regulation pathway. It plays a significant role in the activation of the Ras and Rac small GTPase signaling pathways facilitating changes in cytoskeletal dynamics during cell movement and morphogenesis. Nectin-3 closely associates with other proteins in the Nectin family such as Nectin-1 and Nectin-2 in these pathways enhancing intercellular communication.
Mutations or dysregulation of Nectin-3 have been linked to conditions like mental retardation and developmental abnormalities. It is particularly relevant in the context of intellectual disability where alterations in cell adhesion and signaling can impair proper neuronal connectivity and brain function. Furthermore Nectin-3 interacts with PVRL proteins highlighting its possible involvement in cancer progression and metastasis through altered cell adhesion properties.