Abcam, ab154036, Anti-p53 antibody [9D3DE3]

Size: 100µg
Mouse Recombinant Monoclonal P53 antibody. Suitable for IHC-P, IP, Flow Cyt, WB, In-Cell ELISA, ICC/IF and reacts with Human samples. Cited in 12 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9D3DE3,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow Cyt, In-Cell ELISA, IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This monoclonal antibody to p53 has been knockout validated in Western blot and ICC/IF. The expected signal for p53 was observed in wild type cells and the signal was not seen in knockout cells.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Purification notes-Near homogeneity as judged by SDS-PAGE. ab154036 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation., Storage buffer-pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 0.88% Sodium chloride, 0.36% HEPES, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.