Abcam, ab154961, Anti-MRPL12 antibody

Size: 100µL
Rabbit Polyclonal MRPL12 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MRPL12 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MRPL12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P52815

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPL12 also known as mitochondrial ribosomal protein L12 serves a mechanical function in the mitochondria. MRPL12 is part of the large 39S subunit of the mitochondrial ribosome. The protein has a mass of about 201 kDa and plays a role in protein synthesis by aiding the assembly of ribosomal RNA and proteins. MRPL12 is primarily expressed in tissues with high energy requirements such as the heart liver and skeletal muscle indicating its essential function in energy production processes.
Biological function summary
MRPL12 contributes to mitochondrial ribosome stability and the translation of mitochondrial-encoded proteins. MRPL12 forms interactions within the mitochondrial ribosome complex where it supports ribosomal assembly and function. It works closely with other mitochondrial ribosomal proteins to facilitate the efficient synthesis of proteins important for mitochondrial respiration and energy metabolism. This incorporation into the ribosome complex points to its significance in cellular energy production.
Pathways
MRPL12 is linked to mitochondrial protein synthesis and oxidative phosphorylation pathways. It partners with proteins such as MRPS12 within the ribosomal machinery to ensure the precise translation of mitochondrial mRNA. Its role is critical in maintaining the functional integrity of the electron transport chain which is necessary for ATP production. Proper functioning of these pathways supports cellular energy balance reinforcing the vital role of MRPL12 in cellular metabolism.
MRPL12 has connections to mitochondrial disorders and metabolic syndromes. Altered MRPL12 expression or mutations can impair mitochondrial protein synthesis leading to dysfunctional energy production and contributing to conditions such as mitochondrial myopathy and metabolic encephalopathy. MRPL12 interacts with proteins involved in these diseases such as MRPS12 suggesting a collective impact on mitochondrial function and highlighting the importance of accurate MRPL12 functioning in preventing energy-related disorders.