Abcam, ab155101, Anti-WIF1 antibody [EPR9385]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal WIF1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 24 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9385,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.
Biological function summary
WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.
Pathways
WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.
WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.