Abcam, ab155193, Anti-XYLT2 antibody

Size: 100µL
Rabbit Polyclonal XYLT2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human XYLT2 aa 250-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human XYLT2 aa 250-550. The exact immunogen used to generate this antibody is proprietary information.Q9H1B5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
XYLT2 also known as xylosyltransferase 2 is an enzyme important in the glycosylation process with a molecular weight of approximately 95 kDa. This protein is mainly found in the Golgi apparatus where it participates in the attachment of xylose to specific serine residues within the core protein aiding in the initiation of glycosaminoglycan (GAG) chains. XYLT2's activity contributes to the construction and maintenance of proteoglycans which have a structural and functional role in various cellular matrices.
Biological function summary
XYLT2 operates as an important enzyme in the biosynthesis of glycosaminoglycans primarily contributing to the formation of chondroitin sulfate and heparan sulfate chains. These chains attach to proteoglycans which become part of the extracellular matrix. It doesn’t work as part of a large complex but closely aligns with other enzymes involved in the elongation and modification of GAG chains. The enzyme's activity ensures the proper synthesis and regulation of these macromolecules affecting cellular communication and structural integrity.
Pathways
XYLT2 significantly impacts the GAG biosynthetic pathway. It interacts with proteins like aggrecan and versican which are foundational components of the extracellular matrix. The heparan sulfate biosynthesis pathway also incorporates XYLT2 influencing cellular adhesion and signaling. These pathways play important roles in tissue development and repair as well as in maintaining homeostasis in connective tissues.
XYLT2's activity has connections to disorders such as Ehlers-Danlos syndrome and hereditary multiple exostoses. Mutations or altered expression levels in the XYLT2 gene can lead to abnormal GAG chain formation contributing to these conditions. This relationship involves disturbances in other proteins like EXT1 and EXT2 implicated in cartilage development and maintenance. Understanding XYLT2's role in these disorders can help in diagnosing and potentially developing targeted therapies.