Abcam, ab155433, Human HCC-1 ELISA Kit

Size: 1 x 96Tests
Human HCC-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HCC-1 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 7 pg/mL,
Range:0.006 - 1.5 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's HCC-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay designed for the quantitative measurement of Human HCC-1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human HCC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HCC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HCC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCC-1 also known as CCL14a is a chemokine belonging to the C-C motif chemokine family. This protein has a molecular mass of approximately 10 kDa. HCC-1 is mainly expressed in the liver but can also be found in other tissues like the spleen lung and bone marrow. It plays a direct role in chemotaxis guiding the movement of immune cells to specific sites within the body mainly influencing monocyte activities.
Biological function summary
HCC-1 affects immune response by interacting with chemokine receptors. It binds particularly to CCR1 and CCR5 receptors on the cell surface. Though HCC-1 is not usually part of a larger protein complex it functions closely with other chemokines in modulating leukocyte behavior. These interactions highlight its role in immune surveillance and inflammatory response demonstrating its significance in orchestrating an effective immune defense.
Pathways
HCC-1 is actively involved in chemokine signaling pathways and has a role in inflammation-related processes. Its interaction with CCR1 and CCR5 connects it directly to the MAPK signaling pathway impacting cell proliferation and survival. HCC-1 similar to proteins such as MCP-1 and RANTES plays a part in guiding immune cells during inflammation therefore contributing to the overall coordination of the immune response.
HCC-1 shows a connection with inflammatory diseases and certain types of cancer. Elevated levels of this chemokine associate with rheumatoid arthritis where it may influence disease progression by attracting inflammatory cells to the joints. Additionally HCC-1 has links to liver cancer processes particularly hepatocellular carcinoma due to its high expression in liver tissue. In these contexts proteins like CCR5 are also involved mediating critical pathways that exacerbate disease conditions.