Abcam, ab155554, Anti-Tpit antibody

Size: 100µL
Rabbit Polyclonal Tpit antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBX19 aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TBX19 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.O60806

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tpit also known as TPIT or Tbx19 is a transcription factor that plays a significant role in the differentiation of certain pituitary cells. It has a molecular mass of approximately 62 kDa. Tpit is expressed in the pituitary gland specifically in the cells that produce pro-opiomelanocortin (POMC) which develop into corticotrophs and melanotrophs. Its expression is important for the proper functioning of these endocrine cells directly impacting the hormone production process.
Biological function summary
Tpit acts as a regulator in pituitary development and functioning. It binds to DNA and activates the transcription of POMC leading to the production of hormones such as ACTH (adrenocorticotropic hormone) and MSH (melanocyte-stimulating hormone). Tpit is not a part of a larger complex but functions independently to exert its transcriptional regulation role. Through this mechanism Tpit influences metabolic and stress-related processes in the body.
Pathways
Tpit is deeply involved in the hypothalamic-pituitary-adrenal (HPA) axis a critical stress response system that regulates cortisol production. Tpit regulates POMC expression which in turn affects downstream hormones like ACTH integral to the HPA axis. Moreover its activity is related to other proteins such as CRH (corticotropin-releasing hormone receptor) as they jointly control hormonal signaling pathways. Tpit ensures that the pituitary gland properly synthesizes and secretes hormones in response to various physiological stimuli.
Tpit mutations are associated with disorders such as isolated ACTH deficiency. This disorder results from the failure to produce ACTH leading to adrenal insufficiency and symptoms of fatigue and hypoglycemia. Tpit's role links it to other proteins such as NR5A1 (nuclear receptor subfamily 5 group A member 1) that influence pituitary function and development. Understanding Tpit's involvement in these disorders can help in the development of targeted therapies and improved diagnostic methods in endocrine diseases.