Abcam, ab155899, Free Glycerol Assay Kit II (Colorimetric)

Size: 100Test
Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays.
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue, Suspension cells, Tissue Homogenate, Cell culture supernatant, Serum, Adherent cells,
Assay type:Quantitative,
Sensitivity:< 20 µM,
Range:2 - 10 nmol/well,
Assay time:1h 30m,
Assay Platform:Microplate reader

Product details:
Abcam's Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.
Detection method: colorimetric – based on enzyme activity
This product is manufactured by BioVision, an Abcam company and was previously called K634 Free Glycerol Colorimetric Assay Kit II. K634-100 is the same size as the 100 test size of ab155899.
Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycerol also known as glycerol anhydrous or glycérol is a simple polyol compound with a molecular weight of approximately 92.09 g/mol. Glycerol is expressed widely in various tissues and plays a considerable role as a backbone in the structure of triglycerides and phospholipids thereby impacting lipid biology significantly. It acts mechanically in lipid metabolism by serving as a substrate for glycerolipid synthesis and provides energy through its conversion to glucose or dihydroxyacetone phosphate.
Biological function summary
Glycerol metabolism is significant in energy storage and lipid regulation. It does not function in isolation but is part of various lipid complexes that regulate lipid homeostasis. Through its involvement it aids in the formation of essential lipid components that are important for membrane structure and function. The ability of cells to store energy efficiently is largely dependent on glycerol's capability to integrate into and support these lipid structures.
Pathways
Glycerol participates in glycerolipid metabolism and gluconeogenesis. The glycolytic pathway is especially important as glycerol serves as a potential precursor for glucose production connecting it to energy supply chains within the organism. Proteins like glycerol kinase and glycerol-3-phosphate dehydrogenase play critical roles in these conversions and metabolic processes underlying how glycerol fits into broader biological functions.
Glycerol has connections to metabolic conditions such as glycerol kinase deficiency and nonalcoholic fatty liver disease. Its metabolism's rate and efficiency can influence lipid accumulation and related metabolic syndromes. The enzyme glycerol kinase which phosphorylates glycerol to form glycerol-3-phosphate associates directly with glycerol's relevance in these diseases. Understanding these connections may lead to insights into managing or alleviating related metabolic disease symptoms.