Abcam, ab156034, 10X RIPA Buffer

Size: 15mL
10X RIPA lysis buffer (pH 7.5) (ab156034) is used for cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells and tissues. - Suitable buffer for your western blot SDS-PAGE, ELISA and IP workflow. - Proven performance: cited in over 245 publications.
Key facts
Applications:ELISA, SDS-PAGE, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.5Constituents: 10% Nonylphenol, ethoxylated, 8.8% Sodium chloride, 6.1% Tris, 5% Sodium deoxycholate, 1.12% Sodium pyrophosphate decahydrate, 1% Sodium lauryl sulfate, 0.38% EGTA, 0.29% EDTA, 0.22% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate, 0.18% Sodium orthovanadate

Product details:
Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
Preparation: Dilute to 1X in deionized water
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient