Abcam, ab156035, Native lysis Buffer

Size: 15mL
Native lysis buffer (ab156035) is a ready-to-use buffer for cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells under non-denatured conditions. - Suitable for SDS-PAGE, sELISA, IP and western blot applications.
Key facts
Applications:IP, SDS-PAGE, WB, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.5Constituents: 1.5% Dodecyl maltoside, 0.6% HEPES, 0.58% Sodium chloride, 0.11% Sodium pyrophosphate decahydrate, 0.04% EGTA, 0.03% EDTA, 0.02% Sodium orthovanadate, 0.02% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate

Product details:
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent extracts cytoplasmic, nuclear and membrane proteins. The detergent used in this buffer is relatively gentle and in many cases allows solubilization of protein complexes that retain enzymatic activity. On the flip side, the gentle nature of this lysis buffer means that it may result in extractions that are less complete than lysis buffers using more stringent detergents (e.g.
ab156034
). Native lysis buffer is compatible with many downstream applications, including enzyme activity assays, SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C