Abcam, ab156286, Anti-CRB2 antibody

Size: 100µL
Rabbit Polyclonal CRB2 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CRB2 aa 450-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CRB2 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.Q5IJ48,
Specificity:At least three isoforms of CRB2 are known to exist; ab156286 will detect all three. This antibody is predicted to not crossreact with other members of the CRB family.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CRB2 also known as Crumbs homolog 2 is a protein involved in important cellular functions. It is part of the Crumbs family and has a molecular weight of approximately 137 kDa. Researchers find CRB2 expression largely in the brain and kidneys playing roles in maintaining cell junction integrity. The protein localizes mainly to tight junctions where it plays a mechanically important role in stabilizing cell polarity and tissue organization.
Biological function summary
CRB2 contributes to processes of epithelial cell polarization and morphogenesis. As part of a protein complex involving CRB1 CRB2 and CRB3 it participates in maintaining cell cortex stability. Through this action CRB2 ensures that the cellular architecture remains intact which is essential for proper organ function and development. It influences the formation of apical-basal polarity in epithelial cells which is essential for constructing specialized tissues.
Pathways
CRB2 integrates into the Hippo signaling pathway and tight junction pathway. In the Hippo pathway it closely interacts with proteins like YAP and TAZ to regulate organ size by controlling cell proliferation and apoptosis. By affecting tight junctions CRB2 also influences pathways involved in cell adhesion and the maintenance of cellular barriers connecting with partners such as ZO-1.
CRB2 associates with retinal degeneration and nephrotic syndrome. Mutations affecting its function can lead to retinopathies as its role in maintaining retinal cell structure is compromised. Similarly CRB2's interaction with podocyte proteins is critical in kidney function linking it to nephrotic syndrome when dysfunctional. Pathological conditions also show connections to CRB1 where mutations in both proteins may combine to exacerbate disease states.