Abcam, ab157106, Anti-NAF1 antibody

Size: 100µg
Rabbit Polyclonal NAF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human H/ACA ribonucleoprotein complex non-core subunit NAF1 aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human H/ACA ribonucleoprotein complex non-core subunit NAF1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.Q96HR8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab157106 was affinity purified using an epitope specific to NAF1 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NAF1 also known as NHP2L1 is a protein involved in essential mechanical processes in the cell. It weighs approximately 83 kDa and mainly localizes to the nucleus specifically within Cajal bodies and nucleolus. The protein plays a role in the assembly and maturation of small nucleolar ribonucleoproteins (snoRNPs) which are important for RNA processing. NAF1 expression occurs across diverse tissues indicating its importance in general cellular function.
Biological function summary
NAF1 is a component of the H/ACA ribonucleoprotein complex. It participates in guiding the chemical modification of ribosomal and other small nuclear RNAs. NAF1 acts as a scaffold facilitating the attachment of small RNAs and interacting with other proteins like NOP10 and GAR1 within the snoRNP complex. This organization is essential for the stability and function of the complex which impacts RNA folding and maturation ultimately regulating protein synthesis.
Pathways
The involvement of NAF1 in RNA modification is critical in the RNA splicing and ribosome biogenesis pathways. It plays a role in the maturation of ribosomal RNA an important step in assembling functioning ribosomes. NAF1's activity indirectly influences the translation process with links to additional proteins such as dyskerin which also have essential functions in ribosome assembly and RNA stability.
Studies have connected NAF1 function to conditions like dyskeratosis congenita and certain cancers. The link to dyskeratosis congenita arises from its association with the stability and function of telomerase RNA a pathway shared with proteins such as dyskerin. In some cancers abnormalities in NAF1 expression or function have been implicated in tumor progression highlighting its potential role in maintaining genomic stability and cellular proliferation.