Abcam, ab157457, Anti-OPA1 antibody [EPR11057(B)]

Size: 100µL / 1mL
Anti-OPA1 antibody [EPR11057(B)] (ab157457) is a rabbit monoclonal antibody detecting OPA1 in Western Blot, IHC-P, IHC-Fr . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 60 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11057(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Recent lab testing showed that the antibody detects the band of interest at the proper molecular weight in several cell and tissue lysates. However, the antibody also detects a band at about 50kDa. We could not find in the literature any references on what this band could be. On our test, the 50kDa band detections decreased by decreasing the antibody concentration.

Product details:
What is this antibody validated in?
Anti-OPA1 antibody [EPR11057(B)] (ab157457) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of OPA1?
Anti-OPA1 [EPR11057(B)] (ab157457) specifically detects a band for OPA1 (UniProt: O60313) at a molecular weight of 112kDa.
Trusted by the scientific community
Anti-OPA1 [EPR11057(B)] (ab157457) was first used in a scientific publication in 2013 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-OPA1 [EPR11057(B)] (ab157457) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR11057(B)] also available for your convenience: ab157457, Carrier free -
ab240143
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OPA1 also known as optic atrophy 1 is a dynamin-related GTPase protein important for mitochondrial fusion. OPA1 has a molecular weight of about 120 kDa and is present mostly in the inner mitochondrial membrane. It promotes the maintenance of mitochondrial DNA cristae structures and the modulation of mitochondrial dynamics. Expression of OPA1 occurs in tissues with high energy demands including the retina brain and muscles. Detection of the OPA1 protein can be done using techniques such as Western blot and it reveals different isoforms generated through alternative splicing.
Biological function summary
Mitochondrial dynamics involving OPA1 ensure energy production efficiency and cell health. OPA1 plays a role in mitochondrial fusion by forming a complex with mitofusins MFN1 and MFN2. This complex maintains the integrity of mitochondrial networks facilitates proper respiratory function and prevents apoptosis by regulating cristae junctions. It also participates in the stress response particularly in the preservation of the mitochondrial structure and function under challenging conditions.
Pathways
OPA1 integrates into the mitochondrial fusion and fission pathways important for cellular energy metabolism. It works alongside proteins like DRP1 in balancing these processes. The involvement in these pathways is essential for cellular adaptation to metabolic needs and stress. OPA1 also has a relationship with the PINK1/Parkin pathway where its regulation affects mitophagy a process of clearing damaged mitochondria. These interactions highlight the importance of OPA1 in maintaining cellular and mitochondrial homeostasis.
Mutations in OPA1 have been linked to autosomal dominant optic atrophy and a range of neurodegenerative conditions. The protein's dysfunction leads to the degeneration of the retinal ganglion cells and their axons resulting in vision loss. OPA1 also shows connections to disorders like Charcot-Marie-Tooth disease where its interaction with other proteins like MFN2 plays a role. Deficiency or dysfunction of OPA1 disrupts mitochondrial dynamics leading to cellular energy deficits and contributing to disease pathophysiology.