Abcam, ab157711, Mouse Complement C3 ELISA Kit

Size: 1 x 96Tests
Mouse Complement C3 ELISA Kit ab157711 is a sandwich ELISA used to quantify Mouse Complement Factor 3 (C3) with a sensitivity of 1.65 ng/mL.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.6578 ng/well,
Range:3.13 - 200 ng/mL,
Assay Platform:Microplate

Product details:
Mouse Complement C3 ELISA Kit ab157711 is a sanwich ELISA to measure Mouse Complement factor 3 (C3) in Plasma and Serum with a sensitivity of 1.65 ng/mL.
How the assay works
In this assay the Complement C3 present in samples reacts with the anti- Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay specificity
Interferences:
These chemicals or biologicals will cause interferences in this assay causing compromised results or complete failure.
- Azide and thimerosal at concentrations higher than 0.1% inhibits the enzyme reaction.
Mouse Complement C3 ELISA Kit ab157711 protocol summary
1. Add standards or samples to the wells. Incubate for 20 min
2. Wash, then add 100µl of !x Enzyne-Antibody conjugate per well. Incubate for 20 min
3. Wash, then add 100 µl of TMB solution per well. Incubate 10 min in the dark
4. Add stop solution and read at 450 nm
How other researchers are using Mouse Complement C3 ELISA Kit ab157711
Mouse Complement C3 ELISA Kit ab157711 has been used in publications to evaluate C3 pathways as a therapeutic approach to membranous nephropathy
, to evaluate synthetic RNA viruses as a treatment for cancer
, and to asses CR3 modulation of Asperigillus fumigatus
References:
Nicola M Tomas et al. 2023 PMID: 36709213,
Lorena Lerner et al. 2022 PMID: 36207308,
Li Han et al. 2021 PMID: 34338598
Related and recommended products
Mouse Complement C3 ELISA Kit ab157711 is commonly used to study immune responses against infections as part of the complement system.
Other kits for measuring the complement system include:
- Mouse Complement C5a ELISA kit
ab193718
- Mouse Complement C5 ELISA kit
ab264609
These ELISA kits are also available for human:
- Human Complement C3 ELISA kit
ab108823
- Human Complement C5a ELISA Kit
ab193695
- Human Complement C5 ELISA Kit
ab125963

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement component C3 commonly referred to as C3 or complement 3 is a central protein in the complement system. C3 weighs approximately 185 kDa and is mainly synthesized in the liver. It circulates in the blood plasma in an inactive form. Upon activation C3 is cleaved into two fragments C3a and C3b. C3b plays an important role in opsonization and targeting pathogens for phagocytosis while C3a functions as an anaphylatoxin contributing to the inflammatory response.
Biological function summary
Complement C3 is integral to the immune response by initiating several key processes. It forms part of the C3 convertase complex which is a step within the complement cascade essential for pathogen clearance. Activation of complement C3 leads to the formation of C3b which tags pathogens for destruction by immune cells. It also influences the adaptive immune system providing a bridge to more specific immune responses.
Pathways
Complement C3 plays a part in both the classical and alternative complement pathways. In the classical pathway it interacts with proteins like C4 and C2 to form the C3 convertase complex. In the alternative pathway it is influenced by factor B and factor D allowing C3b to rapidly amplify the response against pathogens. In both pathways C3 is an important focal point to amplify immune response through the generation of C3 convertase enzymes.
Complement C3 dysfunction or dysregulation associates with conditions like age-related macular degeneration and systemic lupus erythematosus. In these diseases improper activation or deficiency of complement C3 exacerbates inflammation and tissue damage. C3b's interaction with protein factors like factor H and I is important to avoid excessive complement activation and imbalances can lead to pathological conditions.