Abcam, ab157731, Rat Complement C3 ELISA Kit

Size: 1 x 96Tests
Rat Complement C3 ELISA Kit is a sandwich ELISA designed to quantify Rat Complement C3 with a sensitivity of 2.82 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 12.5 - 800 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.82 ng/mL,
Range:12.5 - 800 ng/mL,
Assay Platform:Microplate

Product details:
Rat Complement C3 ELISA Kit ab157731 is a sandwich ELISA to measure Rat Complement C3 in serum, plasma with a sensitivity of 2.82 ng/ml.
How the assay works
In this assay the Complement C3 present in samples reacts with the anti-Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Complement C3 antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Rat Complement C3 ELISA Kit ab157731 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
5. Immediately begin recording the color development.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
Biological function summary
Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.
Pathways
The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.
Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.