Abcam, ab157892, Recombinant Human Acyloxyacyl Hydrolase protein

Size: 2µg / 10µg
Recombinant Human Acyloxyacyl Hydrolase protein is a Human Full Length protein, in the 1 to 575 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P28039,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'Acyloxyacyl Hydrolase' also known as AOAH is an enzyme that cleaves secondary acyl chains from the lipid A portion of lipopolysaccharides (LPS) on bacterial membranes decreasing their toxicity. This process is known as hydrolasing activity. AOAH is a hydrolase protein with an approximate mass of 54 kDa and is widely expressed in various cells such as macrophages neutrophils and dendritic cells. Its activity is critical in modulating the immune response to LPS by removing these acyl chains which is essential in preventing excessive inflammation.
Biological function summary
Acyloxyacyl Hydrolase plays a significant role in the detoxification of LPS. As part of the host's defense mechanism AOAH works by reducing the endotoxic effects of LPS which are powerful stimulants of the immune system. Although it is not solely part of a larger protein complex its action significantly impacts the innate immune response. This enzyme ensures that the immune system does not overreact to bacterial invasion which could otherwise lead to harmful inflammatory responses.
Pathways
Acyloxyacyl Hydrolase functions within the Toll-like receptor (TLR) signaling pathway which is instrumental in recognizing LPS and initiating an immune response. AOAH modifies LPS diminishing its ability to trigger TLR4 a receptor that responds to the presence of bacterial LPS. By doing so it regulates signaling pathways involved in innate immunity. Additionally it has a relationship with proteins like myeloid differentiation factor 88 (MyD88) an important adaptor protein in the TLR signaling cascade which mediates inflammatory responses.
Acyloxyacyl Hydrolase has connections to sepsis and chronic inflammatory conditions. In sepsis the clearance of LPS by AOAH helps mitigate excessive inflammation reducing the severity of the condition. Hospitals often face challenges in managing sepsis due to overwhelming immune responses where AOAH’s role in detoxifying LPS is critical. Moreover in chronic inflammatory diseases AOAH's activity serves as a checkpoint maintaining inflammation at manageable levels. Its relationship with TLR4 highlights its role in controlling pathways that drive such disorders.