Abcam, ab158343, Recombinant Human Elastase-1 protein

Size: 10µg
Recombinant Human Elastase-1 protein is a Human Full Length protein, in the 1 to 258 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9UNI1,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as CELA1.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Elastase-1 also known as ELA1 or pancreatic elastase functions as a serine protease. This enzyme with a molecular mass of approximately 29 kDa assists in breaking down elastin an important protein in the connective tissue. Elastase-1 primarily expresses in the pancreas and gets secreted into the duodenum where it plays an important role in digestion. Its specificity for insoluble elastin makes it distinct among proteolytic enzymes.
Biological function summary
Elastase-1 contributes significantly to digestive processes by degrading elastin and other proteins in the small intestine. This action prepares proteins for absorption into the body. Elastase-1 does not work alone; it acts as part of a complex group of pancreatic enzymes including trypsin and chymotrypsin which together facilitate complete protein digestion. These associated enzymes jointly ensure efficient breakdown of dietary components optimizing nutrient uptake.
Pathways
Elastase-1 participates in the digestion and absorption pathways of proteins. It functions alongside other digestive enzymes such as amylase and lipase which are essential in the breakdown of carbohydrates and fats. Elastase-1 has connections to pathways involved in proteolysis acting synergistically with enzymes like trypsin for effective protein digestion. These pathways highlight the cooperative nature of enzyme systems in the digestive tract.
Elastase-1 often becomes a focus in conditions such as chronic pancreatitis and cystic fibrosis. Reduced levels of elastase-1 in the stool serve as a marker for pancreatic insufficiency a condition where the pancreas fails to secrete adequate digestive enzymes. In cystic fibrosis thick mucus secretions can impede elastase-1 from reaching the duodenum exacerbating malabsorption issues. Proteins like trypsin and chymotrypsin can also be involved as impaired pancreatic enzyme excretion affects multiple enzyme systems complicating nutrient absorption and digestion.